P-142: Effects of Main Substrate of Nitric Oxide in The First Half of Pregnancy onTestis in Mice Embryos and Off spring

Background: Nitric oxide (NO) is an important molecular regulator in biology of reproductive systems. In addition, critical level of NO is essential for Implantation and embryo’s development. However, increased levels of NO can have a wide range of adverse effects. This molecule is synthesized from L-Arginine (L.A) by Nitric oxide Synthetase (NOS) activity. In the current study, the effects of increased dose of L.A in pregnant mice on their embryo and offspring’s testis tissue have been investigated. Materials and Methods: Twenty-four female NMRI mice were selected and randomly divided into three groups of eight upon observing the vaginal plaque that has been considered day zero in pregnancy. L.A was used at a dose of 200 ml/kg (i.p.) for three days respectively on 3rd, 4th, and 5th days of pregnancy. One day before and seven days after delivery, histological changes in testis were assessed by Histotechnique method and HandE staining. Results: Our data showed that using L.A treatment in pregnant mice is led to reduced number of seminiferous tubules and decreased diameter Tunica albuginea in embryos that were dissected one day before delivery in comparison with that of control and normal saline groups. In addition to the mentioned changes, although they were more severe in embryos, dissemination was observed in offspring group that were dissected seven days after their birth. Conclusion: The results suggest that excess dose on NO generated by L.A induction in blood of pregnant mice, affect testis tissue and structure in their embryos and offspring, that can interfere with normal function of the mentioned organ. However, these changes seem to be improved during their growth, which needs more experiments.